yrkC [2019-01-02 15:22:29]

mother cell-specific sporulation protein

locus: BSU26560

pI: 7.35

mw: 21.11 kDa

protein length: 186 aa

gene length: 558 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 2,714,933 → 2,715,493

The protein

[SW|Domains]

• [SW|cupin 2 domain] (aa 89 ... 164)

Structure

• [PDB|2OA2] (the [SW|cupin 2 domain], from B. halodurans, 66% identity)

Expression and Regulation

Operons

• genes: [gene|BBF2D58DFC1E345BB841AFDFEF5E651715B1426B|yrkC]-[gene|B97A503AA247375DCA28009A7D3895F6327BE3F5|yrzN]
  description: [Pubmed|22383849,12480901]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,12480901], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]) [Pubmed|15699190,12480901]

Biological materials

Mutant

• MGNA-C456 (yrkC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2454 NBRP B. subtilis, Japan]
• BKE26560 (Δ[gene|BBF2D58DFC1E345BB841AFDFEF5E651715B1426B|yrkC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTGATCCCCGCAAT, downstream forward: _UP4_TAGCACAGATATTCTGGAGA
• BKK26560 (Δ[gene|BBF2D58DFC1E345BB841AFDFEF5E651715B1426B|yrkC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTGATCCCCGCAAT, downstream forward: _UP4_TAGCACAGATATTCTGGAGA

References

• 15699190,12480901